The EMBO Journal Table of Contents for 15 December 2017; Vol. 36, No. 24

15 December 2017 | Volume 36, Number 24 Submit Table of Contents News & Views Articles Volume 36, Number 24 News & Views Cohesin: building loops, but not compartments Recent work demonstrates that cohesin loading is essential for the 3D organization of vertebrate genomes via active loop extrusion. Judith HI Haarhuis and Benjamin D Rowland Published online 07.12.2017 Don't judge a cell by its cover: heterogeneity within early lymphoid progenitors Single‐cell RNA sequencing reveals a mouse lymphoid progenitor population with presumed multilineage potential to consist of cells with distinct genetic lineage signatures. Göran Karlsson, Mikael Sigvardsson and Charlotta Böiers Published online 30.11.2017 Articles An invisible ubiquitin conformation is required for efficient phosphorylation by PINK1 Unmodified ubiquitin protein exists in an equilibrium of two distinct conformational states, whose mutational stabilization differentially affects conjugation and phosphorylation reactions. Christina Gladkova, Alexander F Schubert, Jane L Wagstaff, Jonathan N Pruneda, Stefan MV Freund and David Komander Published online 13.11.2017 Topologically associating domains and chromatin loops depend on cohesin and are regulated by CTCF, WAPL, and PDS5 proteins Hi‐C data show that cohesin is required for long‐range chromatin interactions, supporting a model of loop extrusion driving the spatial organization of mammalian genomes. Gordana Wutz, Csilla Várnai, Kota Nagasaka, David A Cisneros, Roman R Stocsits, Wen Tang, Stefan Schoenfelder, Gregor Jessberger, Matthias Muhar, M Julius Hossain, Nike Walther, Birgit Koch, Moritz Kueblbeck, Jan Ellenberg, Johannes Zuber, Peter Fraser and Jan‐Michael Peters Published online 07.12.2017 A mechanism of cohesin‐dependent loop extrusion organizes zygotic genome architecture Single‐nucleus Hi‐C and microscopy show that the zygotic genome folds into loops and domains in a cohesin‐dependent manner with differences in maternal and paternal chromosome organization. Johanna Gassler, Hugo B Brandão, Maxim Imakaev, Ilya M Flyamer, Sabrina Ladstätter, Wendy A Bickmore, Jan‐Michael Peters, Leonid A Mirny and Kikuë Tachibana Published online 07.12.2017 Single‐cell RNA sequencing reveals developmental heterogeneity among early lymphoid progenitors A mouse hematopoietic precursor population with presumed multilineage potential is on the single‐cell level composed of cells with distinct lymphoid and myeloid genetic signatures. Llucia Alberti‐Servera, Lilly von Muenchow, Panagiotis Tsapogas, Giuseppina Capoferri, Katja Eschbach, Christian Beisel, Rhodri Ceredig, Robert Ivanek and Antonius Rolink Published online 13.10.2017 Mutational signatures of non‐homologous and polymerase theta‐mediated end‐joining in embryonic stem cells DNA double‐strand break configuration dictates the choice between two distinct error‐prone repair pathways in mouse embryonic stem cells, resulting in different repair outcomes. Joost Schimmel, Hanneke Kool, Robin van Schendel and Marcel Tijsterman Published online 27.10.2017 Tumor suppressor Tsc1 is a new Hsp90 co‐chaperone that facilitates folding of kinase and non‐kinase clients Besides stabilizing Tsc2 and forming the mTOR‐regulating TSC complex, Tsc1 plays a more general role as a co‐chaperone for Hsp90 on a range of client proteins. Mark R Woodford, Rebecca A Sager, Elijah Marris, Diana M Dunn, Adam R Blanden, Ryan L Murphy, Nicholas Rensing, Oleg Shapiro, Barry Panaretou, Chrisostomos Prodromou, Stewart N Loh, David H Gutmann, Dimitra Bourboulia, Gennady Bratslavsky, Michael Wong and Mehdi Mollapour Published online 10.11.2017 The E3 ubiquitin ligase APC/CCdh1 degrades MCPH1 after MCPH1‐βTrCP2‐Cdc25A‐mediated mitotic entry to ensure neurogenesis Neurogenesis is governed by microcephalin (MCPH1) degradation by APC/CCdh1 and its regulation of βTrCP2‐mediated Cdc25A degradation during the cell cycle. Xiaoqian Liu, Wen Zong, Tangliang Li, Yujun Wang, Xingzhi Xu, Zhong‐Wei Zhou and Zhao‐Qi Wang Published online 17.11.2017   Unsubscribe from or edit your subscription for this service. Or by mail: Customer Service * 973 University Avenue * Los Gatos, CA 95032 * U.S.A. Copyright © 2017 by the European Molecular Biology Organisation.